Leaf protoplasts containing chloroplasts

Leaf protoplasts containing chloroplasts

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Caption: Light micrograph of a group of protoplasts from a leaf. A protoplast is a single intact cell, which has been chemically treated to remove the tough outer cellulose wall, leaving only the delicate plasma membrane binding the cell contents into a whole. The cell wall can later be regrown. The cells contain chloroplasts (green), the sites of photosynthesis. Protoplasts, lacking the tough wall, are easier to manipulate. They are used in plant genetic engineering, including work on hybridization & disease resistance. The techniques developed by protoplast technology are pertinant also to biotechnology & medicine.

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Keywords: botanical science, botany, cell, cells, chloroplast, dna microinjection, genetic engineering (plant), light micrograph, lm, nuclear fusion (cell), plant structure, plasma membrane, protoplast

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