Fused protoplasts from tobacco leaf cells

Fused protoplasts from tobacco leaf cells

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Caption: Scanning electron micrograph showing fusion of a pair of protoplasts from two tobacco leaf cells, Nicotiana tabacum (cultivar White Burley). A protoplast is a single intact cell which has been chemically treated to remove the tough outer cellulose wall, leaving only the delicate plasma membrane binding the cell contents together. Chemical stimulation causes the wall to regrow. Protoplast technology has enabled two types of genetic experimentation; nuclear fusion (seen here), where the cytoplasm & nuclei of two cells are chemically encouraged to fuse, & DNA microinj- ection, where selected genes are injected directly into a cell. Mag: X330 (35mm), X2600 (10x8-inch).

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Keywords: biotechnology, botanical science, botany, cell, cells, genetic engineering, nuclear fusion, plant structure, plasma membrane, protoplast, scanning electron micrograph, sem

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