Thin layer chromatogram

Thin layer chromatogram

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Credit: SINCLAIR STAMMERS/SCIENCE PHOTO LIBRARY.

Caption: Thin layer chromatogram (TLC) of an extract of thylakoid membranes from the leaf of annual meadow grass Poa annua. TLC plastic sheets are coated with a 60 F254 silica gel which measures 0.2 millimetres thick. Three drops of extract, corresponding to the three columns here, were laid at the bottom of the sheet. The sheet was then placed in a beaker of solvent (75% acetone & 25% petroleum ether). The picture shows the solubility of the extract in solvent. Seven bands are seen; top (orange) is carotene; 2 (green) pheophytin; 3 (green) chlorophyll A; 4 (green) chlorophyll B; 5,6 & 7 are carotenoids. The line crossing the top of image is the solvent line.

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Keywords: botanical science, botany, carotene, carotenoid, cell, chlorophyll, chloroplast, chloroplasts, chromatogram, photosynthesis, plant structure, plastid, plastids, solubility in solvent, thin layer chromatogram, thylakoid

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