Culture of phage M13, vector of DNA for Sry gene

Culture of phage M13, vector of DNA for Sry gene

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Credit: PHILIPPE PLAILLY/SCIENCE PHOTO LIBRARY

Caption: Culture colony of the bacteriophage M13, used as a source of DNA fragments containing the Sry (sex- determining region Y) gene, the switch that redirects mammalian development from its usual pathway - female - to male. Joint research by the Medical Research Council & Imperial Cancer Research Fund showed that some normal, chromosomally-female mice embryos developed as males with testes following their injection with a small 14-kilobase fragment of DNA containing Sry. The DNA for Sry was released from its phage vector by digestion with a restriction enzyme, isolated by gel electrophoresis (part of the autoradiogram is shown here) & then purified.

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Keywords: bacteriophage, biochemistry, biotechnology, dna research, dna sequencing, gene, genetic, genetics, m13, m13 bacteriophage, m13 phage and, mice, mouse, mouse sex, phage m13, sex-determining gene, sry, sry dna, sry dna and, sry gene, sry gene sequence, testis-determining gene

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