Preparing electrophoresis gel for DNA sequencing

Preparing electrophoresis gel for DNA sequencing

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Credit: PETER MENZEL/SCIENCE PHOTO LIBRARY

Caption: Technician using gel electrophoresis to prepare a DNA sequencing autoradiogram, a technique used to examine the base-pair sequence or chemical blueprint of lengths of DNA. The two strands of nucleotides of which DNA is composed are joined by base pairing; adenine (A) always pairs with thymine (T), guanine (G) with cytosine (C). Hence the base sequence defines a section of DNA. Decoding this sequence is done by electrophoresis through an agarose gel, where fragments of radioactively-tagged DNA separate according to size. The banding pattern (fluorescent pink) is revealed under ultraviolet light. A hard copy is obtained by blotting with radiographic film.

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Keywords: biochemistry, biotechnology, blue, dna, dna lab, dna research, dna sequencing autoradiography, electrophoresis gel, gel electrophoresis, genetic, genetics, laboratory, researcher, scientist, sequencing, woman

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