Southern blotting

Southern blotting

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Credit: JAMES KING-HOLMES/SCIENCE PHOTO LIBRARY

Caption: Southern blotting. A researcher transfers an electrophoresis gel containing DNA into a tray of salt solution prior to blotting the DNA onto nitrocellulose paper. The purple dye shows how far electrophoresis has progressed. Southern blotting is a technique to reveal specific fragments of DNA (deoxyribonucleic acid) in a complex mixture. The total DNA is cut into fragments and separated according to size by gel electrophoresis. The DNA is then blotted onto a sheet of nitrocellulose placed on top, and a radioactive DNA probe added to the nitrocellulose. The resulting banding pattern, revealed by autoradiography, can be used to map the structure of particular genes.

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Keywords: agarose gel, biochemistry, biotechnology, blot, blotting, dna, dna lab, dna research, gel, gel electrophoresis, gene mapping, genetic, genetic research, genetics, laboratory, southern, southern blo, southern blot

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