Two Dimensional Electrophoresis

Two Dimensional Electrophoresis

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Credit: EDWARD KINSMAN/SCIENCE PHOTO LIBRARY

Caption: MODEL RELEASED. A researcher studies data from a 2 dimensional electrophoresis experiment. 2 dimensional electrophoresis (2DE) is one of the best experimental tools for the reliable separation of thousands of proteins in a single gel. 2DE consists of a tandem pair of electrophoretic separations. In the first dimension, proteins are resolved according to their isoelectric points. In the second dimension, proteins are separated according to their approximate molecular weight using sodium dodecyl sulfate poly acrylamide electrophoresis. This technique can provide molecular weight approximations for most proteins.

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