Reverse Transcription Polymerase Chain Re

Reverse Transcription Polymerase Chain Re

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Credit: DNA ILLUSTRATIONS/SCIENCE PHOTO LIBRARY

Caption: This illustration shows the process of reverse transcription polymerase chain reaction (RT-PCR). a double stranded DNA is transcribed via tRNA that binds to the PB site on the mRNA strand. As the first DNA strand is formed the mRNA strand degrades. Once the second complementary strand of DNA is formed from the last remaining PP site of the mRNA, the double stranded DNA is formed. This is the reverse transcription (RT) phase. Then the polymerase chain reaction (PCR) portion starts. In the Initiation and denaturation phase, the double stranded DNA is separated by heat which degrades the hydrogen bonds attaching the two strands. With subsequent cooling, the primers and polymerase are able to bind to the single strands of DNA which allow the complementary

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Keywords: annealing, artwork, cgi, denaturation, digitally generated, dna cloning, double stranded dna, elongation, gene profiling, graphic, illustration, medical illustration, mrna, polymerase chain reaction, primer, reverse transcription

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