Nonlinear microscopy

Nonlinear microscopy

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Caption: Nonlinear microscopy. Researchers using a two-photon excitation microscope to image living tissue (images on screen at left). Different cells within the tissue have been labelled with different fluorescent dyes. An infrared laser beam is focused through the objective lens. As it hits the fluorescent dyes it excites them, causing them to fluoresce. The laser emits pulses that last for only femtoseconds (one millionth of one billionth of a second). This imaging technique is able to penetrate deep into biological tissue and image entire networks of cells.

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