CRISPR-CAS9 gene editing complex

CRISPR-CAS9 gene editing complex

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Caption: CRISPR-CAS9 gene editing complex. Computer illustration showing the molecular structure of the CRISPR-associated protein Cas9 in complex with guide RNA (ribonucleic acid, red) and target DNA (deoxyribonucleic acid, orange-fuchsia) containing a canonical 5'-NGG-3' PAM (yellow). Cas9 is a nuclease protein associated with the CRISPR (Clustered Regularly Interspersed Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes and other bacteria. It uses a guide RNA sequence to cut DNA at a complementary site. Seen here: Alpha-helical lobe (grey), HNH domain (blue), RuvC domain (cyan), C-terminal-domain (pink), topo homology domain (pale pink), arginine-rich helix (fuchsia), guideRNA (red), target DNA (orange), non-target DNA (fuchsia).

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