Virology laboratory

Virology laboratory

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Caption: VIROLOGY LABORATORY Photo essay from laboratory. Institut Pasteur. Department of virology. Unit of molecular genetics of Bunyaviridae. Group entomology (Mosquito team). Research on the transmission of Chikungunya virus to mosquitoes Aedes albopictus and Aedes aegypti. Here, titration of the Chikungunya virus by counting the lysis plaques formed on the Vero cells. The Vero epithelial cell line is issue from the kidney of a vervet monkey (Chlorocebus aethiops) ; it is used in the studies of viral replication and cell lysis. A suspension of the virus is added to the cells in culture ; during the incubation, each viral particle lysis the cell it has infected. After coloration of the cells with violet crystal, the count of the lysis plaques that have formed enable to determine the concentration in virus of the suspension.

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