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Plant cell plasmolysis, light microscopy


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Upper epidermis of an red onion skin undergoing plasmolysis, followed by deplasmolysis. The cells contain vacuoles with red anthocyanin. The footage starts with normal cells called 'turgid', that is they are pretty full of water and hence there is enough pressure against the rigid cell wall for the cells to keep their brick-like shape. An 1mol potassium nitrate solution is added under the cover slide, water is drawn out of the cell, the vacuoles and the protoplasts shrink. In some cells you can see Hechtian strands, thread-like strands connecting the cell wall to the plasmalemma. Then water is added to the slide and the concentration of water in the vacuoles increase with the protoplasts pushed against the cell walls, the cells are turgid again. Footage taken with time-lapse of about 500% with an 20x objective, field of view 0,5mm, magnification 200x when printed 10 centimetres wide.

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  • Duration: 00:00:48
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