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DNA fingerprinting process

DNA fingerprinting process

G210/0194

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Credit

MICHAEL GILBERT / SCIENCE PHOTO LIBRARY MICHAEL GILBERT / SCIENCE PHOTO LIBRARY

Caption

The DNA fingerprinting process. Blood sample. DNA is extracted from blood cells. DNA is cut into fragments by a restriction enzyme. The DNA fragments are separated into bands during electrophoresis in an agarose gel. The DNA band pattern in the gel is transferred to a nylon membrane. Radioactive DNA probe, designed to recognise specific DNA sequences, is prepared. Probe DNA binds to the specific DNA sequences on the membrane. Excess DNA is washed off, but the DNA pattern is invisible at this stage. X-ray film is placed next to the membrane & exposed to the radioactive DNA. The developed X-ray shows the pattern of DNA bands - the DNA fingerprint.

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