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Culture of phage M13, vector of DNA for Sry gene

Culture of phage M13, vector of DNA for Sry gene

G210/0328

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Credit

PHILIPPE PLAILLY / SCIENCE PHOTO LIBRARY PHILIPPE PLAILLY / SCIENCE PHOTO LIBRARY

Caption

Culture colony of the bacteriophage M13, used as a source of DNA fragments containing the Sry (sex- determining region Y) gene, the switch that redirects mammalian development from its usual pathway - female - to male. Joint research by the Medical Research Council & Imperial Cancer Research Fund showed that some normal, chromosomally-female mice embryos developed as males with testes following their injection with a small 14-kilobase fragment of DNA containing Sry. The DNA for Sry was released from its phage vector by digestion with a restriction enzyme, isolated by gel electrophoresis (part of the autoradiogram is shown here) & then purified.

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