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False-colour SEM of a cluster of hybridoma cells

False-colour SEM of a cluster of hybridoma cells

G400/0044

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Credit

DAVID SCHARF / SCIENCE PHOTO LIBRARY DAVID SCHARF / SCIENCE PHOTO LIBRARY

Caption

False-colour scanning electron micrograph of a cluster of hybridoma cells used in the production of monoclonal antibodies. Monoclonal antibodies are produced by injecting a mouse with an antigen & harvesting its antibody response by removing the B-lymphocytes. These are then fused with myeloma (tumour) cells taken from a mutant lymphocyte source. The fusion product is called a hybridoma cell. B lymphocytes are short-lived, but when fused with tumour cells, which divide indefinitely, the continuous production of antibody is secured. The hybridoma cell is cloned & produces a single type of antibody. Magnification x1,600 at 6x6 cm size.

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