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Human neutrophils, laser microscopy

K004/0556

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CHAD T. ROBINSON / SCIENCE PHOTO LIBRARY CHAD T. ROBINSON / SCIENCE PHOTO LIBRARY

Caption

Human neutrophils. Scanning laser microscopy of human neutrophils digesting Acinetobacter baumannii bacteria (rod-shaped). Neutrophils are the most abundant white blood cell in humans. They are part of the immune system; engulfing and digesting invading bacteria. The reactive oxygen species (ROS) indicator dihydrorhodamine 123 (DHR) has been added to these cells via passive diffusion. The neutrophils produce oxygen ions and hydrogen peroxide in order to destroy the bacterial cell walls. These ROS molecules oxidize the indicator to hydrorhodamine which fluoresces yellow when stimulated with the laser light from the microscope.

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