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Thin-layer chromatography

Thin-layer chromatography

C025/9052

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33.8 x 22.6 cm ⏐ 13.3 x 8.9 in (300dpi)

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Credit

TREVOR CLIFFORD PHOTOGRAPHY / SCIENCE PHOTO LIBRARY TREVOR CLIFFORD PHOTOGRAPHY / SCIENCE PHOTO LIBRARY

Caption

Thin-layer chromatography (TLC) plate in a beaker, showing separated components (colours). TLC is a chromatography technique used to separate non-volatile mixtures. It is performed on a sheet of glass, plastic, or aluminium foil coated in a thin layer of adsorbent material (the stationary phase). The sample is applied to the plate and a solvent (the mobile phase) is drawn up the plate via capillary action. Different components move up the plate at different rates, causing them to separate, as shown here.

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