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Chromatography

Chromatography

C036/3412

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Credit

CHARLES D. WINTERS / SCIENCE PHOTO LIBRARY CHARLES D. WINTERS / SCIENCE PHOTO LIBRARY

Caption

Chromatography is the collective term for a set of laboratory techniques for the separation of mixtures. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus changing the separation.

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