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Indirect immunofluorescence test, illustration

Indirect immunofluorescence test, illustration

F020/1952

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58.4 MB (1.4 MB compressed)

6000 x 3400 pixels

50.8 x 28.7 cm ⏐ 20.0 x 11.3 in (300dpi)

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Credit

KATERYNA KON / SCIENCE PHOTO LIBRARY KATERYNA KON / SCIENCE PHOTO LIBRARY

Caption

Mechanism of indirect immunofluorescence test, computer illustration. Immunofluorescence is a cell imaging technique based on the use of antibodies to label a specific target antigen (bacteria, cancer cells, other) with a fluorescent dye (also called fluorophore or fluorochrome). The fluorescent dye allows visualization of the antigen distribution in the sample under a fluorescent microscope. Indirect immunofluorescence uses two antibodies, the primary and the secondary. The primary antibody directed against the target antigen is unconjugated, while the secondary antibody is conjugated with the fluorophore and is directed against the primary antibody.

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